Data schema#
This document defines the standard for data acquired with waveorder.
Raw directory organization#
Currently, we structure raw data in the following hierarchy:
working_directory/ # commonly YYYY_MM_DD_exp_name, but not enforced
├── polarization_calibration_0.txt
│ ...
├── polarization_calibration_<i>.txt # i calibration repeats
│
├── bg_0
│ ...
├── bg_<j> # j background repeats
│ ├── background.zarr
│ ├── polarization_calibration.txt # copied into each bg folder
│ ├── reconstruction.zarr
│ ├── reconstruction_settings.yml # for use with `waveorder reconstruct`
│ └── transfer_function.zarr # for use with `waveorder apply-inv-tf`
│
├── <acq_name_0>_snap_0
├── <acq_name_0>_snap_1
│ ├── raw_data.zarr
│ ├── reconstruction.zarr
│ ├── reconstruction_settings.yml
│ └── transfer_function.zarr
│ ...
├── <acq_name_0>_snap_<k> # k repeats with the first acquisition name
│ ├── raw_data.zarr
│ ├── reconstruction.zarr
│ ├── reconstruction_settings.yml
│ └── transfer_function.zarr
│ ...
│
├── <acq_name_l>_snap_0 # l different acquisition names
│ ...
├── <acq_name_l>_snap_<m> # m repeats for this acquisition name
├── raw_data.zarr
├── reconstruction.zarr
├── reconstruction_settings.yml
└── transfer_function.zarr
Each .zarr contains an OME-NGFF v0.4 in HCS format with a single field of view.